For the single sample, this cut-off had sensitivity, specificity and likelihood ratio for S. aureus was isolated had CMT scores > or = 2 this would classify 51.6% of the 940 quarter-samples as positive. Eighty-two percent of the samples in which S. Using a CMT score of > or = 1 would classify 78.6% of the 940 quarter-samples as positive. aureus were positive for two of two, and for at least two of the first three consecutive quarter-milk samples collected from that cow, respectively. aureus subclinical mastitis if results of microbiologic culture for S. For the duplicate and triplicate quarter-samples, cows were considered positive for S. All samples were screened for subclinical mastitis by the CMT as well as microbiological culture of single, duplicate (two consecutive) and triplicate (three consecutive) samples as a gold standard. A total of 1151 of quarter-milk samples were CMT tested, of these 914-originated from cows with a lactation period of 14-305 days. The current study was undertaken to investigate the clinical utility of CMT for screening of Staphylococcus aureus subclinical mastitis in low yielding smallholder dairy cows in Tanzania. However, given the innately high physiological level of somatic cells in low yielding dairy cows, this threshold may not apply to low yielding dairy cows. CMT score of > or = 1 corresponding to > or = 500,000 somatic cells ml(-1) is commonly used as threshold of subclinical mastitis in temperate countries. Screening of subclinical mastitis under field conditions is done using the California mastitis test (CMT).

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